验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HEK-293 cells, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19928-1-AP targets CEP72 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13905 Product name: Recombinant human CEP72 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 299-647 aa of BC001750 Sequence: HPDSMDTEDSASSQKLDLSGEMVPGPLPAPGKCRKRRMPVGRFQTFSDQEGLGCPERTHGSSVPKESLSRQDSSESRNGRTLSQPEASETEEQRSRGVTDTREPSPGSHSALPGKKTALQAALLETLLDLVDRSWGGCRSLHSNEAFLAQARHILSSVEEFTAAQDSSAMVGEDVGSLALESKSLQSRLAEQQQQHAREMSEVTAELHHTHKELDDLRQHLDKSLEENSRLKSLLLSMKKEVKSADTAATLNLQIAGLQTSVKRLCGEIVELKQHLEHYDKIQELTQMLQESHSSLVSTNEHLLQELSQVRAQHRAEVEQMHWSYQELKKTMALFPHSSASHGGCQAC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 72kDa |
| 别名 | KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa |
| 计算分子量 | 646 aa, 72 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC001750 |
| 基因名称 | CEP72 |
| Gene ID (NCBI) | 55722 |
| RRID | AB_10638607 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P209 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP72 antibody 19928-1-AP | Download protocol |
| IP protocol for CEP72 antibody 19928-1-AP | Download protocol |
| WB protocol for CEP72 antibody 19928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
| ||
EMBO Rep Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. | ||
J Cell Sci Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. | ||
Sci Rep Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.
| ||
J Biol Chem Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131). | ||
BMC Biol CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72 |
