验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CEP192 Ready-To-Use IHC Kit. CEP192 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18832-1-AP targets CEP192 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 192kDa |
| 别名 | centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein |
| 计算分子量 | 213 kDa |
| 观测分子量 | 250-300 kDa |
| GenBank蛋白编号 | BC144481 |
| 基因名称 | CEP192 |
| Gene ID (NCBI) | 55125 |
| RRID | AB_2878560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CEP192 antibody 18832-1-AP | Download protocol |
| IF protocol for CEP192 antibody 18832-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
