验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27325-1-AP targets CEP170 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag26311 Product name: Recombinant human CEP170 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1012-1202 aa of BC140794 Sequence: EASDSELADADKASVASEVSTTSSTSKPPTGRRNISRIDLLAQPRRTRLGSLSARSDSEATISRSSASSRTAEAIIRSGARLVPSDKFSPRIRANSISRLSDSKVKSMTSAHGSASVNSRWRRFPTDYASTSEDEFGSNRNSPKHTRLRTSPALKTTRLQSAGSAMPTSSSFKHRIKEQEDYIRDWTAHRE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 170kDa |
| 别名 | centrosomal protein 170kDa, Centrosomal protein of 170 kDa, CEP170, FAM68A, KAB, KARP 1 binding protein, KARP1 binding protein, KIAA0470 |
| 计算分子量 | 1486 aa, 165 kDa |
| 观测分子量 | 170-190 kDa |
| GenBank蛋白编号 | BC140794 |
| 基因名称 | CEP170 |
| Gene ID (NCBI) | 9859 |
| RRID | AB_2880844 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SW79 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP170 antibody 27325-1-AP | Download protocol |
| WB protocol for CEP170 antibody 27325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
J Cell Biol DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair. | ||
Elife α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.
| ||
Reprod Biomed Online A novel CCDC39 mutation causes multiple morphological abnormalities of the flagella in a primary ciliary dyskinesia patient |
