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验证数据展示

  • WB analysis using 68715-1-Ig (same clone as 68715-1-PBS)

    WB analysis using 68715-1-Ig (same clone as 68715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68715-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68715-1-Ig (same clone as 68715-1-PBS)

    IF Staining of HeLa using 68715-1-Ig (same clone as 68715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68715-1-PBS in a different storage buffer formulation.

CEP135 Monoclonal antibody, PBS Only

CEP135 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68715-1-PBS

产品说明书

CloneNo. 3C4B7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68715-1-PBS targets CEP135 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19122

Product name: Recombinant human CEP135 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-233 aa of BC136535

Sequence: MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrosomal protein 135kDa
别名 CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7
计算分子量 1140 aa, 134 kDa
观测分子量135 kDa
GenBank蛋白编号BC136535
基因名称 CEP135
Gene ID (NCBI) 9662
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ66GS9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

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