CEP135 Polyclonal antibody

CEP135 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (1)

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24428-1-AP

Print datasheet

Synonyms

centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635



经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, U2OS cells, U-87 MG cells
Positive IF detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, Canine
Cited Reactivityhuman, mouse
Immunogen CEP135 fusion protein Ag19122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 135kDa
Synonyms centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635
Calculated Molecular Weight 1140 aa, 134 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC136535
Gene Symbol CEP135
Gene ID (NCBI) 9662
RRIDAB_2879543
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ66GS9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

实验方案

Product Specific Protocols
WB protocol for CEP135 antibody 24428-1-APDownload protocol
IF protocol for CEP135 antibody 24428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Curr Biol

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Authors - Hindol Gupta
mouseIF

EMBO Rep

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Authors - Ting Song
human

Elife

WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.

Authors - Emmanuelle Steib
humanIF

Cells

Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.

Authors - Zhenzhen Chu
humanIF

Mol Biol Cell

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.

Authors - Alexander J Stemm-Wolf
humanIF

Gene

A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).

Authors - Yan-Wei Sha