验证数据展示
产品信息
86805-3-PBS targets CENPE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27230 Product name: Recombinant human CENPE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2401-2500 aa of NM_001286734 Sequence: MESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | centromere protein E, 312kDa |
| 别名 | Kinesin 7, CENP E, Centromere associated protein E, Centromere protein E, Kinesin related protein CENPE |
| 计算分子量 | 316 kDa |
| 观测分子量 | 300-316 kDa |
| GenBank蛋白编号 | NM_001286734 |
| 基因名称 | CENPE |
| Gene ID (NCBI) | 1062 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q02224 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Centromeric protein E (CENPE) is also named Kinesin-7. CENPE, a member of the centromere protein (CENP) family, functions as a microtubule plus-end directed motor protein that is upregulated during the G2 phase (PMID: 31115500, PMID: 7851898, PMID: 38560520). It plays a crucial role in the spindle assembly checkpoint (SAC), chromosome alignment, and microtubule capture at kinetochores during mitosis (PMID: 30784092). CENPE is essential for mitotic white matter, which accumulates during the G2 phase of the cell cycle, is consumed throughout mitosis and degrades later in the cell cycle (PMID: 33862458). CENPE regulates the cell cycle by targeting the transcription of CDK1 in the nucleus (PMID: 33862458). Studies on tumor cells have shown that CENPE plays an important role in the regulation of cell proliferation, apoptosis, migration and other activities (PMID: 33862458).
