验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IHC | See 3 publications below |
产品信息
28142-1-AP targets CENPE in WB, IHC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human |
文献引用反应性 | human, mouse |
免疫原 | CENPE fusion protein Ag27230 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centromere protein E, 312kDa |
别名 | CENP E, CENPE, Centromere protein E, centromere protein E, 312kDa, KIF10, Kinesin related protein CENPE |
计算分子量 | 316 kDa |
观测分子量 | 300-316 kDa |
GenBank蛋白编号 | NM_001286734 |
基因名称 | CENPE |
Gene ID (NCBI) | 1062 |
RRID | AB_2881072 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02224 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CENPE antibody 28142-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Mol Cell Res Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes. | ||
Am J Transl Res Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma. | ||
PLoS One CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival. | ||
Cell Mol Life Sci Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse | ||
Transl Oncol A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity | ||
J Cancer Development and Validation of the novel Cuproptosis- and Immune-related Signature for Predicting Prognosis in Hepatocellular Carcinoma |