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验证数据展示

  • WB analysis using 87672-1-RR (same clone as 87672-1-PBS)

    WB analysis using 87672-1-RR (same clone as 87672-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87672-1-RR (CENPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87672-1-PBS in a different storage buffer formulation.

  • WB analysis using 87672-1-RR (same clone as 87672-1-PBS)

    WB analysis using 87672-1-RR (same clone as 87672-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87672-1-RR (CENPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87672-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 87672-1-RR (same clone as 87672-1-PBS)

    WB analysis of HeLa using 87672-1-RR (same clone as 87672-1-PBS)

    WB result of CENPB antibody (87672-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPB transfected HeLa cells. This data was developed using the same antibody clone with 87672-1-PBS in a different storage buffer formulation.

CENPB Recombinant monoclonal antibody, PBS Only

CENPB Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87672-1-PBS

产品说明书

CloneNo. 253126C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

CENP B, CENP-B, Centromere protein B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87672-1-PBS targets CENPB in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24967

Product name: Recombinant human CENPB protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-180 aa of BC053847

Sequence: MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 centromere protein B, 80kDa
别名 CENP B, CENP-B, Centromere protein B
计算分子量 65 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC053847
基因名称 CENPB
Gene ID (NCBI) 1059
RRIDAB_3745687
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07199
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Centromere protein B (CENPB), also known as major centromere autoantigen B, is an autoantigen protein of the cell nucleus. In mammalian centromeres CENPB plays a critical role in the organization of chromatinstructure, but in the absence of CENPB some chromosomes use other redundant proteins to accomplish mitosis (PMID: 14963831). CENPB plays an important role in cell cycle regulation. CENPB is highly expressed in cancer cells helping in high rate proliferation. This antibody detects CENPB protein with a molecular weight of 80 kDa (PMID: 34547289, PMID: 29273057).

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