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验证数据展示

  • IHC staining of mouse brain using 19940-1-AP

    IHC staining of mouse brain using 19940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19940-1-AP (CELSR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 19940-1-AP

    IF Staining of SH-SY5Y using 19940-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CELSR2 antibody (19940-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CELSR2 Polyclonal antibody

CELSR2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 19940-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

EGF-like protein 2, EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19940-1-AP targets CELSR2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
别名 EGF-like protein 2, EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10
计算分子量 318 kDa
GenBank蛋白编号NM_001408
基因名称 CELSR2
Gene ID (NCBI) 1952
RRIDAB_3669318
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCU4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may have an important role in cell/cell signaling during nervous system formation.

实验方案

Product Specific Protocols
IHC protocol for CELSR2 antibody 19940-1-APDownload protocol
IF protocol for CELSR2 antibody 19940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols