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验证数据展示

  • WB analysis using 85081-4-RR

    WB analysis using 85081-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85081-4-RR (CEL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 85081-4-RR

    IHC staining of human pancreas using 85081-4-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 85081-4-RR

    IHC staining of human pancreas using 85081-4-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85081-4-RR

    Affinity and Kinetic Characterization of 85081-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85081-4-RR against Human CEL were performed. The affinity constant is 2.46 nM.

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CEL Recombinant monoclonal antibody

CEL Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85081-4-RR

产品说明书

CloneNo. 242761B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Bile salt-activated lipase, 242761B11, BAL, Bile salt-stimulated lipase, BSDL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85081-4-RR targets CEL in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5262

Product name: Recombinant human CEL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 371-722 aa of BC042510

Sequence: SEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGDSGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carboxyl ester lipase (bile salt-stimulated lipase)
别名 Bile salt-activated lipase, 242761B11, BAL, Bile salt-stimulated lipase, BSDL
计算分子量 79 kDa
观测分子量79 kDa
GenBank蛋白编号BC042510
基因名称 CEL
Gene ID (NCBI) 1056
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19835
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CEL antibody 85081-4-RRDownload protocol
IHC protocol for CEL antibody 85081-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols