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验证数据展示

  • WB analysis of human liver using 15384-1-AP (same clone as 15384-1-PBS)

    WB analysis of human liver using 15384-1-AP (same clone as 15384-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

    IF Staining of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CEL Polyclonal antibody, PBS Only

CEL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 15384-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15384-1-PBS targets CEL in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5262

Product name: Recombinant human CEL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 371-722 aa of BC042510

Sequence: SEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGDSGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxyl ester lipase (bile salt-stimulated lipase)
别名
计算分子量 79 kDa
观测分子量 67 kDa
GenBank蛋白编号BC042510
基因名称 CEL
Gene ID (NCBI) 1056
RRIDAB_2229297
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19835
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

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