验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23895-1-AP targets CEBPD in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | CEBPD fusion protein Ag20953 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CCAAT/enhancer binding protein (C/EBP), delta |
| 别名 | C/EBP delta, C/EBPdelta, CELF, CRP3 |
| 计算分子量 | 269 aa, 28 kDa |
| 观测分子量 | 28-36 kDa |
| GenBank蛋白编号 | BC105109 |
| 基因名称 | CEBPD |
| Gene ID (NCBI) | 1052 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P49716 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEBPD, a versatile transcription factor, belongs to the C/EBP family that comprises three parts: an N-terminal transactivating region, a basic DNA binding region, and a C-terminal leucine zipper domain (PMID: 17170124). It presents as a regulator in terms of cell differentiation, proliferation, motility, growth arrest, and cell death (PMID: 24155666). The apparent molecular weight is larger than the calculated molecular weight of 28 kDa, which is likely due to posttranslational modification, presumably by ubiquitination.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEBPD antibody 23895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |