• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse colon using 24626-1-AP

    WB analysis of mouse colon using 24626-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24626-1-AP

    IHC staining of human colon using 24626-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24626-1-AP (CEACAM7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CEACAM7 Polyclonal antibody

CEACAM7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24626-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Carcinoembryonic antigen CGM2, CEA, CEACAM7, CGM2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24626-1-AP targets CEACAM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18579

Product name: Recombinant human CEACAM7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-213 aa of BC121132

Sequence: VTHNDAGIYTLHVIKENLVNEEVTRQFYVFSEPPKPSITSNNFNPVENKDIVVLTCQPETQNTTYLWWVNNQSLLVSPRLLLSTDNRTLVLLSATKNDIGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 7
别名 Carcinoembryonic antigen CGM2, CEA, CEACAM7, CGM2
计算分子量 265 aa, 29 kDa
观测分子量58 kDa
GenBank蛋白编号BC121132
基因名称 CEACAM7
Gene ID (NCBI) 1087
RRIDAB_3085742
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14002
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM7, also known as CGM2, belongs to the carcinoembryonic antigen (CEA) family. CEACAM7 contains three domains: an N-terminal IgV domain, a single IgC2 domain, and a cell-surface GPI anchor domain. CEACAM7 achieves cell adhesion through dimerization of the N-terminal IgV domain. CEACAM7 is expressed on highly differentiated epithelial cells of the colon and rectum and on the epithelial cells within the ducts of the pancreas (PMID: 26323304). CEACAM7 has 2 isoforms with the molecular mass of 19 and 29 kDa. The observed MW of CEACAM7 is 58 kDa, which is consistent with the description in the literature (PMID: 36828119).

实验方案

Product Specific Protocols
WB protocol for CEACAM7 antibody 24626-1-APDownload protocol
IHC protocol for CEACAM7 antibody 24626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols