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验证数据展示

  • WB analysis of HeLa using 17209-1-AP

    WB analysis of HeLa using 17209-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17209-1-AP

    IF Staining of HeLa using 17209-1-AP

    Immunofluorescent analysis of Hela cells, using CEACAM21 antibody 17209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CEACAM21 Polyclonal antibody

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17209-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Carcinoembryonic antigen-related cell adhesion molecule 21, UNQ3098/PRO10075

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CEACAM21 fusion protein Ag10992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 21
别名 Carcinoembryonic antigen-related cell adhesion molecule 21, UNQ3098/PRO10075
计算分子量191aa,22 kDa; 293aa,32 kDa
观测分子量 32 kDa, 22 kDa
GenBank蛋白编号BC012001
基因名称 CEACAM21
Gene ID (NCBI) 90273
RRIDAB_2077481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KPI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21) is a member of the CEACAM family that has diverse functions in cell adhesion, in intracellular and intercellular signaling, and during complex biological processes such as cancer progression, inflammation, angiogenesis, and metastasis (PMID: 23903773; 32488569). CEACAM21 acts as a heterophilic ligand for TIM-3, leading to the dissociation of Bat3 and increasing proximal TCR signaling and proapoptotic protein expression in late effector T cells, thereby mediating T cell suppression (PMID: 38490257).

实验方案

Product Specific Protocols
WB protocol for CEACAM21 antibody 17209-1-APDownload protocol
IF protocol for CEACAM21 antibody 17209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols