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验证数据展示

  • WB analysis using 31519-1-AP

    WB analysis using 31519-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 31519-1-AP (CEA/CD66e antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 31519-1-AP

    IHC staining of human colon cancer using 31519-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31519-1-AP (CEA/CD66e antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colon cancer using 31519-1-AP

    IHC staining of human colon cancer using 31519-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31519-1-AP (CEA/CD66e antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included

CEA/CD66e Polyclonal antibody

CEA/CD66e Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31519-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CEACAM5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31519-1-AP targets CEA/CD66e in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 5
别名 CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CEACAM5
计算分子量 77 kDa
观测分子量200 kDa
GenBank蛋白编号NM_004363
基因名称 CEA
Gene ID (NCBI) 1048
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP06731-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

实验方案

Product Specific Protocols
IHC protocol for CEA/CD66e antibody 31519-1-APDownload protocol
WB protocol for CEA/CD66e antibody 31519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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