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CDX2 Polyclonal antibody

CDX2 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30380-1-AP

Print datasheet

Synonyms

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX3, Homeobox protein CDX 2

验证数据展示

  • WB analysis using 30380-1-AP

    WB analysis using 30380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30380-1-AP (CDX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 30380-1-AP

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 30380-1-AP

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse colon using 30380-1-AP

    IHC staining of mouse colon using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of rat colon using 30380-1-AP

    IHC staining of rat colon using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 30380-1-AP

    IF Staining of Caco-2 using 30380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (30380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human colon cancer using 30380-1-AP

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included

经过测试的应用

Positive WB detected inmouse colon tissue, pig colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30380-1-AP targets CDX2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen CDX2 fusion protein Ag32826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caudal type homeobox 2
Synonyms caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX3, Homeobox protein CDX 2
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

实验方案

Product Specific Protocols
WB protocol for CDX2 antibody 30380-1-APDownload protocol
IHC protocol for CDX2 antibody 30380-1-APDownload protocol
IF protocol for CDX2 antibody 30380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols