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验证数据展示

  • WB analysis using 21655-1-AP

    WB analysis using 21655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 21655-1-AP

    WB analysis using 21655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of Caco-2 using 21655-1-AP

    IP experiment of Caco-2 using 21655-1-AP

    IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.

  • IHC staining of human colon cancer using 21655-1-AP

    IHC staining of human colon cancer using 21655-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX1 antibody included
  • WB analysis of Caco-2 using 21655-1-AP

    WB analysis of Caco-2 using 21655-1-AP

    WB result of CDX1 antibody (21655-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDX1 transfected Caco-2 cells.

  • IHC staining of mouse colon using 21655-1-AP

    IHC staining of mouse colon using 21655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX1 antibody included
  • IF Staining of Caco-2 using 21655-1-AP

    IF Staining of Caco-2 using 21655-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • KD/KO Validated

CDX1 Polyclonal antibody

CDX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21655-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Caudal type homeobox 1, Caudal type homeobox protein 1, Caudal-type homeobox protein 1, Homeobox protein CDX 1, Homeobox protein CDX-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, mouse small intestine tissue, mouse stomach tissue, mouse colon tissue, rat colon tissue
Positive IP detected inCaco-2 cells
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy CDX1 Ready-To-Use IHC Kit. CDX1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21655-1-AP targets CDX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16376

Product name: Recombinant human CDX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 105-154 aa of BC096252

Sequence: KQQQQQPPQPPMAHDITATPAGPSLGGLCPSNTSLLATSSPMPVKEEFLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caudal type homeobox 1
别名 Caudal type homeobox 1, Caudal type homeobox protein 1, Caudal-type homeobox protein 1, Homeobox protein CDX 1, Homeobox protein CDX-1
计算分子量 265 aa, 28 kDa
观测分子量 28-35 kDa
GenBank蛋白编号BC096252
基因名称 CDX1
Gene ID (NCBI) 1044
RRIDAB_2878901
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47902
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CDX1 antibody 21655-1-APDownload protocol
IHC protocol for CDX1 antibody 21655-1-APDownload protocol
IP protocol for CDX1 antibody 21655-1-APDownload protocol
WB protocol for CDX1 antibody 21655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Biol Int

Piwei Peiyuan Pill Ameliorates Chronic Atrophic Gastritis and Intestinal Metaplasia at Least in Part by Inhibiting the Pellino1/IRAK1/NF-κB Signaling Axis.

Authors - Cheng Zhang
View Article
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