验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, NIH/3T3 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13184-1-AP targets CDSN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3854 Product name: Recombinant human CDSN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-503 aa of BC031993 Sequence: NSYRGILNPSQPGQSSSSSQTFGVSSSGQSVSSNQRPCSSDIPDSPCSGGPIVSHSGPYIPSSHSVSGGQRPVVVVVDQHGSGAPGVVQGPPCSNGGLPGKPCPPITSVDKSYGGYEVVGGSSDSYLVPGMTYSKGKIYPVGYFTKENPVKGSPGVPSFAAGPPISEGKYFSSNPIIPSQSAASSAIAFQPVGTGGVQLCGGGSTGSKGPCSPSSSRVPSSSSISGSSGLPYHPCGSASQSPCSPPGTGSFSSSSSSQSSGKIILQPCGSKSSSSGHPCMSVSSLTLTGGPDGSPHPDPSAGAKPCGSSSAGKIPCRSIRDILA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | corneodesmosin |
| 别名 | S protein, HTSS, D6S586E, corneodesmosin |
| 计算分子量 | 528 aa, 52 kDa |
| 观测分子量 | 55-65 kDa |
| GenBank蛋白编号 | BC031993 |
| 基因名称 | CDSN |
| Gene ID (NCBI) | 1041 |
| RRID | AB_11183052 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15517 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDSN, also named as S protein, is important for the epidermal barrier integrity. CDSN is a unique extracellular 52- to 56-kDa glycoprotein found in corneodesmosomes. Pathogenic mutations in CDSN have been linked in humans to genodermatoses affecting both the hair follicle and the epidermis. Inflammatory peeling skin syndrome is caused a novel mutation in CDSN.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDSN antibody 13184-1-AP | Download protocol |
| WB protocol for CDSN antibody 13184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Sphingosine 1-Phosphate Receptor 2 Is Central to Maintaining Epidermal Barrier Homeostasis. | ||
iScience OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules | ||
Exp Dermatol Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion. | ||
Clin Cosmet Investig Dermatol Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model. | ||
Heliyon Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer
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