验证数据展示
经过测试的应用
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CDR2L Ready-To-Use IHC Kit. CDR2L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14563-1-AP targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | CDR2L fusion protein Ag6127 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cerebellar degeneration-related protein 2-like |
| 别名 | Paraneoplastic 62 kDa antigen, HUMPPA, Cerebellar degeneration-related protein 2-like |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC047534 |
| 基因名称 | CDR2L |
| Gene ID (NCBI) | 30850 |
| RRID | AB_2076740 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86X02 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IHC protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IF protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IP protocol for CDR2L antibody 14563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis. | ||
Cancer Immunol Immunother Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies. | ||
Ann Clin Transl Neurol Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration. | ||
J Neuroimmunol Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue. | ||
bioRxiv CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization
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