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验证数据展示

  • WB analysis using 16615-1-AP

    WB analysis using 16615-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16615-1-AP

    IHC staining of human kidney using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HeLa using 16615-1-AP

    IP experiment of HeLa using 16615-1-AP

    IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

  • IF Staining of HepG2 using 16615-1-AP

    IF Staining of HepG2 using 16615-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDKN2AIP antibody (16615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CDKN2AIP Polyclonal antibody

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16615-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

CDKN2A-interacting protein, CARF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16615-1-AP targets CDKN2AIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 CDKN2AIP fusion protein Ag9907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDKN2A interacting protein
别名 CDKN2A-interacting protein, CARF
计算分子量 579 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC022270
基因名称 CDKN2AIP
Gene ID (NCBI) 55602
RRIDAB_2244775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IHC protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IF protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IP protocol for CDKN2AIP antibody 16615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Cell Biosci

CDKN2AIP is critical for spermiogenesis and germ cell development

Authors - Yuming Cao
View Article
humanWB

Clin Cosmet Investig Dermatol

Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.

Authors - Xianhong Yang
View Article