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验证数据展示

  • WB analysis using RMX00038

    WB analysis using RMX00038

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00038 (CDKN2A/P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using RMX00038

    WB analysis using RMX00038

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00038 (CDKN2A/P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using RMX00038

    IHC staining of human cervical cancer using RMX00038

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using RMX00038 (CDKN2A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN2A/P16INK4A antibody included
  • IF Staining of HeLa using RMX00038

    IF Staining of HeLa using RMX00038

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDKN2A antibody (RMX00038) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® CDKN2A/P16-INK4A Multi-Recombinant antibody

CDKN2A/P16-INK4A Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00038

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CDKN2A, P16 INK4A, P16-INK4A, CDK4I, CDKN2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HeLa cells, TF-1 cells, C6 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00038 targets CDKN2A/P16-INK4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 2A
别名 CDKN2A, P16 INK4A, P16-INK4A, CDK4I, CDKN2
计算分子量 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC021998
基因名称 CDKN2A
Gene ID (NCBI) 1029
RRIDAB_3741448
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP42771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. The third one -p14(ARF) is entirely unrelated. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma.

实验方案

Product Specific Protocols
IF protocol for CDKN2A/P16-INK4A antibody RMX00038Download protocol
IHC protocol for CDKN2A/P16-INK4A antibody RMX00038Download protocol
WB protocol for CDKN2A/P16-INK4A antibody RMX00038Download protocol
Standard Protocols
Click here to view our Standard Protocols
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