验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
13888-1-AP targets NKIAMRE in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4812 Product name: Recombinant human CDKL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-279 aa of BC041799 Sequence: MEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase-like 3 |
| 别名 | CDKL3, cyclin dependent kinase like 3, NKIAMRE |
| 计算分子量 | 592 aa, 68 kDa |
| 观测分子量 | 52-60 kDa |
| GenBank蛋白编号 | BC041799 |
| 基因名称 | NKIAMRE |
| Gene ID (NCBI) | 51265 |
| RRID | AB_2077463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVW4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily.This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NKIAMRE antibody 13888-1-AP | Download protocol |
| WB protocol for NKIAMRE antibody 13888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol CDKL3 Targets ATG5 to Promote Carcinogenesis of Esophageal Squamous Cell Carcinoma. | ||
Front Physiol Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3.
|
