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验证数据展示

  • WB analysis of HeLa using 13888-1-AP

    WB analysis of HeLa using 13888-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13888-1-AP

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13888-1-AP

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NKIAMRE Polyclonal antibody

NKIAMRE Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13888-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CDKL3, cyclin dependent kinase like 3, NKIAMRE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13888-1-AP targets NKIAMRE in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 NKIAMRE fusion protein Ag4812 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase-like 3
别名 CDKL3, cyclin dependent kinase like 3, NKIAMRE
计算分子量 592 aa, 68 kDa
观测分子量 52-60 kDa
GenBank蛋白编号BC041799
基因名称 NKIAMRE
Gene ID (NCBI) 51265
RRIDAB_2077463
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily.This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.

实验方案

Product Specific Protocols
WB protocol for NKIAMRE antibody 13888-1-APDownload protocol
IHC protocol for NKIAMRE antibody 13888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

CDKL3 Targets ATG5 to Promote Carcinogenesis of Esophageal Squamous Cell Carcinoma.

Authors - Suna Zhou
View Article
humanWB,IHC

Front Physiol

Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3.

Authors - Jinduo Zhang
  • KD Validated
View Article