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  • KD/KO Validated

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)


FC, IF, IHC, IP, WB, ELISA and More (1)



Cat No : 14052-1-AP

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CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE


Positive WB detected inHEK-293 cells, Jurkat cells, C2C12 cells, HeLa cells, MOLT-4 cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human urothelial carcinoma tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells


Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery


14052-1-AP targets CDK6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat, zebrafish
Immunogen CDK6 fusion protein Ag5275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 6
Synonyms CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a 

protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition, 

thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to 

release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication 

(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell 

cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions, 

where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.

What is the molecular weight of CDK6?

The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).

What is the subcellular localization of CDK6?

CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are 

located in the nuclei of proliferating cells.

What is the expression pattern of CDK6?

CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and 

members of the INK4 family of CDK inhibitors (PMID: 7739547).

What is CDK6's role in cancer?

CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately 

80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the 

hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation 

of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6 

expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).


Product Specific Protocols
WB protocol for CDK6 antibody 14052-1-APDownload protocol
IHC protocol for CDK6 antibody 14052-1-APDownload protocol
IF protocol for CDK6 antibody 14052-1-APDownload protocol
IP protocol for CDK6 antibody 14052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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