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  • Featured Product
  • KD/KO Validated

CDK5RAP3 Polyclonal antibody

CDK5RAP3 Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11007-1-AP

Print datasheet

Synonyms

C53, CDK5RAP3, HSF 27, IC53, LZAP, MST016, OK/SW cl.114, PP1553, Protein HSF 27

验证数据展示

  • WB analysis using 11007-1-AP

    WB analysis using 11007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • WB analysis of HEK-293 using 11007-1-AP

    WB analysis of HEK-293 using 11007-1-AP

    WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IHC staining of human pancreas using 11007-1-AP

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IHC staining of human pancreas using 11007-1-AP

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IF Staining of human liver using 11007-1-AP

    IF Staining of human liver using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 11007-1-AP

    IF Staining of HepG2 using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 11007-1-AP

    FC experiment of HepG2 using 11007-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CDK5RAP3 antibody (11007-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HEK-293T cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human liver tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11007-1-AP targets CDK5RAP3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse, bovine
Immunogen CDK5RAP3 fusion protein Ag1474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDK5 regulatory subunit associated protein 3
Synonyms C53, CDK5RAP3, HSF 27, IC53, LZAP, MST016, OK/SW cl.114, PP1553, Protein HSF 27
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC009957
Gene Symbol CDK5RAP3
Gene ID (NCBI) 80279
RRIDAB_2076869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

实验方案

Product Specific Protocols
WB protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IHC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IF protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
FC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,bovineWB

Int J Mol Sci

CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.

Authors - Yangyang Shen
  • KD Validated
View Article