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  • Featured Product
  • KD/KO Validated

CDK5RAP1 Polyclonal antibody

CDK5RAP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14740-1-AP

Print datasheet

Synonyms

C20orf34, C42, CDK5RAP1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167

验证数据展示

  • WB analysis using 14740-1-AP

    WB analysis using 14740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 14740-1-AP

    IHC staining of human pancreas using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse brain using 14740-1-AP

    IHC staining of mouse brain using 14740-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inC6 cells, human heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14740-1-AP targets CDK5RAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CDK5RAP1 fusion protein Ag6409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDK5 regulatory subunit associated protein 1
Synonyms C20orf34, C42, CDK5RAP1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC050706
Gene Symbol CDK5RAP1
Gene ID (NCBI) 51654
RRIDAB_2076843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SZ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK5RAP1 antibody 14740-1-APDownload protocol
IHC protocol for CDK5RAP1 antibody 14740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

METTL17 is an Fe-S cluster checkpoint for mitochondrial translation

Authors - Tslil Ast
  • KD Validated
View Article