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验证数据展示

  • WB analysis using 84749-1-RR (same clone as 84749-1-PBS)

    WB analysis using 84749-1-RR (same clone as 84749-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 84749-1-RR (same clone as 84749-1-PBS)

    IP experiment of mouse brain using 84749-1-RR (same clone as 84749-1-PBS)

    IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

    IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5 antibody included
  • IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

    IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5 antibody included
  • IF Staining of SH-SY5Y using 84749-1-RR (same clone as 84749-1-PBS)

    IF Staining of SH-SY5Y using 84749-1-RR (same clone as 84749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (84749-1-RR, Clone: 241908F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

CDK5 Recombinant monoclonal antibody, PBS Only

CDK5 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 84749-1-PBS

产品说明书

CloneNo. 241908F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

241908F10, Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.

产品信息

84749-1-PBS targets CDK5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28465

Product name: Recombinant human CDK5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 92-273 aa of BC005115

Sequence: DSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 5
别名 241908F10, Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1
计算分子量 31 kDa, 33 kDa
观测分子量 31-33 kDa
GenBank蛋白编号BC005115
基因名称 CDK5
Gene ID (NCBI) 1020
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ00535
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

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