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验证数据展示

  • WB analysis using 83994-3-RR (same clone as 83994-3-PBS)

    WB analysis using 83994-3-RR (same clone as 83994-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

    IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

    IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)

    IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • IHC staining of human cervical squamous cancer using 83994-3-RR (same clone as 83994-3-PBS)

    IHC staining of human cervical squamous cancer using 83994-3-RR (same clone as 83994-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included

CDK4 Recombinant antibody, PBS Only

CDK4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 83994-3-PBS

产品说明书

CloneNo. 241053F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

产品信息

83994-3-PBS targets CDK4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 CDK4 fusion protein Ag20538 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 4
别名 EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4
计算分子量 34 kDa
观测分子量30-34 kDa
GenBank蛋白编号BC010153
基因名称 CDK4
Gene ID (NCBI) 1019
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11802
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).