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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CDK4 (66950-1-Ig, Clone:1G2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

CDK4 Monoclonal antibody, PBS Only

CDK4 Monoclonal Antibody for ELISA, IHC, WB
Cat No. 66950-1-PBS

产品说明书

CloneNo. 1G2C12

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

ELISA, IHC, WB

别名

PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

产品信息

66950-1-PBS targets CDK4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 ELISA, IHC, WB Application Description
经测试反应性 rat, mouse, human
免疫原

CatNo: Ag20538

Product name: Recombinant human CDK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-303 aa of BC010153

Sequence: MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 4
别名 PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC010153
基因名称 CDK4
Gene ID (NCBI) 1019
RRIDAB_2882273
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11802
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).