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验证数据展示

  • WB analysis using 30461-1-AP (same clone as 30461-1-PBS)

    WB analysis using 30461-1-AP (same clone as 30461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30461-1-AP (CDK13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 30461-1-AP (same clone as 30461-1-PBS)

    FC experiment of K-562 using 30461-1-AP (same clone as 30461-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDK13 (30461-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 30461-1-AP (same clone as 30461-1-PBS)

    IHC staining of human ovary cancer using 30461-1-AP (same clone as 30461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30461-1-AP (CDK13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK13 antibody included
  • IF Staining of HeLa using 30461-1-AP (same clone as 30461-1-PBS)

    IF Staining of HeLa using 30461-1-AP (same clone as 30461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK13 antibody (30461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

CDK13 Polyclonal antibody, PBS Only

CDK13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 30461-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CDC2L5, CHED, CDC2L, CDC2 related protein kinase 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK13 Ready-To-Use IHC Kit. CDK13 primary antibody included.

产品信息

30461-1-PBS targets CDK13 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32897

Product name: Recombinant human CDK13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1150-1300 aa of NM_003718.4

Sequence: QESSKPLGGIQPSSQTIQPKVETDAAQAAVQSAFAVLLTQLIKAQQSKQKDVLLEERENGSGHEASLQLRPPPEPSTPVSGQDDLIQHQDMRILELTPEPDRPRILPPDQRPPEPPEPPPVTEEDLDYRTENQHVPTTSSSLTDPHAGVKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 2-like 5 (cholinesterase-related cell division controller)
别名 CDC2L5, CHED, CDC2L, CDC2 related protein kinase 5
计算分子量165 kDa
观测分子量 170 kDa
GenBank蛋白编号NM_003718.4
基因名称 CDK13
Gene ID (NCBI) 8621
RRIDAB_3086325
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase 13 (CDK13) is also named as CDC2L, CDC2L5, CHED and KIAA1791, and belongs to the CMGC Ser/Thr protein kinase family. Interestingly, in TNBC cells silencing CDK13 provokes cell death without affecting DDR genes. Like CDK12, CDK13 phosphorylates the Ser2 position of the heptad repeat in the CTD of RNA Pol II (PMID:26748711). CDK13/CycK complex is that regulates transcription by phosphorylating serine residues at positions 5 and 2 of the heptapeptide repeats (Y-S-P-T-S-P-S) comprising the C-terminal domain (CTD) of RPB1, the largest subunit of RNA polymerase II (RNAPII). CDK13 is expressed in fetal brain, liver, and muscle, and adult whole brain, hippocampus, and bone marrow (PMID:30904094).

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