CDK10 Polyclonal antibody

CDK10 Polyclonal Antibody for IHC, ELISA
Cat No. 17182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA and More (1)

CDK10, cyclin dependent kinase 10, PISSLRE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDK10 Ready-To-Use IHC Kit. CDK10 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17182-1-AP targets CDK10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10935

Product name: Recombinant human CDK10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-283 aa of BC017342

Sequence: MDKEKDGIPISSLREITLLLRLRHPNIVELKEVVVGNHLESIFLVMGYCEQDLASLLENMPTPFSEAQVKCIVLQVLRGLQYLHRNFIIHRDLKVSNLLMTDKGCVKTADFGLARAYGVPVKPMTPKVVTLWYRAPELLLGTTTQTTSIDMWAVGCILAELLAHRPLLPGTSEIHQIDLIVQLLGTPSENIWPGFSKLPLVGQYSLRKQPYNNLKHKFPWLSEAGLRLLHFLFMATAGDCLESSYFKEKPLPCEPELMPTFPHHRNKRAAPATSEGQSKRCKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 10
别名 CDK10, cyclin dependent kinase 10, PISSLRE
计算分子量 283 aa, 32 kDa
观测分子量 38 kDa
GenBank蛋白编号BC017342
基因名称 CDK10
Gene ID (NCBI) 8558
RRIDAB_2878356
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15131
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 10 (CDK10) is a Cdc2-related kinase that was discovered based on its homology to the Cdc2 PSTA1RE amino acid domain (PMID: 8208557). CDK10 plays a pivotal role in the regulation of fundamental cellular processes, including cell proliferation, transcription regulation, and cell cycle regulation. It partners with cyclin M to phosphorylate substrates such as ETS2 and PKN2 in order to modulate cellular growth. Initial reports have indicated that CDK10 may act as a tumor suppressor in breast cancer (PMID: 26392360). Additional studies have demonstrated tumor suppressive and oncogenic roles for CDK10 in malignancies such as hepatobiliary cancers, gastric cancer, glioma, nasopharyngeal carcinoma, and colorectal cancer(PMID: 22326270,PMID: 29512714, PMID: 23740091, PMID: 29845196)

实验方案

Product Specific Protocols
IHC protocol for CDK10 antibody 17182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-433 accelerates acquired chemoresistance of gallbladder cancer cells by targeting cyclin M.

Authors - Jianhua Yu
humanWB

BMB Rep

Downregulated CDK10 promotes cancer progression and radioresistance in lung cancer through activating the JNK/c-Jun signaling pathway

Authors - Chaojin Hong
  • KD Validated
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