验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, rat skin tissue, rat colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87514-4-RR targets CDCP1/CD318 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg6199 Product name: Recombinant mouse CD318/CDCP1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-666 aa of NM_133974.3 Sequence: SEIALPQRSGVTVSIKLGNPALPVKICYIVMSRQHITELIIRPGERKSFTFSCSNPEKHFVLKIEKNIDCMSGPCPFGEVHLQPSTSELPILNRTFIWDVRAHKSIGLELQFATPRLRQIGPGESCADGVTHSISGHIDATEVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHRRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPGGFPEDELMTWQFVVPAHLRASVSFLNFNVSNCERKEERVEYYIPGSTTNPEVFRLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQRPQDESNKTYMVDLSRERTMSLTIEPRPVKHGRRFVPGCFVCLESRTCSTNVTLTAGSIHKISFLCDDLTRLWVNVEKTLSCLDHRYCYRQSFKLQVPDYILQLPVQLHDFSWKLLVPKDKLSLMLVPGQKLQQHTQERPCNTSFGYHVTSTTPGQDLYFGSFCSGGSIEKIQVKQNSSVTLRAYAPSFQQEVSKQGLIVSYTPYFKEEGIFTVTPDTKNKVYLRSPNWDRGLPALSSVSWNISVPSNQVACLTVLKERSGLACQSGRAFMIIQEQQSRAEEIFSLEEEVLPKPSFHHHSFWVNISNCSPMNGKQLDLLFWVTLTPRTVDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CUB domain containing protein 1 |
| 别名 | CD318, Cdcp1, CUB domain-containing protein 1, Membrane glycoprotein gp140, Transmembrane and associated with src kinases |
| 计算分子量 | 93 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | NM_133974.3 |
| 基因名称 | Cdcp1 |
| Gene ID (NCBI) | 109332 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5U462 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD318 (also known as CDCP1, TRASK, SIMA135, or gp140) is a cell-surface glycoprotein with an apparent molecular mass of ∼140 kDa. It consists of three extracellular CUB domains, a transmembrane domain, and an intracellular domain. CD318 can be proteolytically cleaved between the two distal CUB domains by certain serine proteases, resulting in different ratios of the ∼140-kDa intact molecule and the ∼80-kDa cleaved product on various cells. Cleaved CD318 is phosphorylated and activated by Src kinase, then the activated CD318 forms a complex with activated β1 integrin and activates FAK/PI3K/Akt motility signaling to promote early tumor dissemination. Under normal conditions, CD318 is present on many epithelial cells, some hematopoietic cells (18), and mesenchymal stem cells. CD318 is also present on many tumor cells. Up-regulation of CD318 expression is associated with a poor prognosis for many cancer patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCP1/CD318 antibody 87514-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
