CDCP1 Polyclonal antibody

CDCP1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12754-1-AP

Print datasheet

Synonyms

CD318, CDCP1, Membrane glycoprotein gp140, SIMA135, TRASK



经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, PC-3 cells
Positive FC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12754-1-AP targets CDCP1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CDCP1 fusion protein Ag3439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CUB domain containing protein 1
Synonyms CD318, CDCP1, Membrane glycoprotein gp140, SIMA135, TRASK
Calculated Molecular Weight 836 aa, 93 kDa
Observed Molecular Weight 72 kDa, 130 kDa
GenBank Accession NumberBC021099
Gene Symbol CDCP1
Gene ID (NCBI) 64866
RRIDAB_2078799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5V8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDCP1, also named as CD318, TRASK and SIMA 135, is a 135-140 kDa transmembrane protein that contains three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

实验方案

Product Specific Protocols
WB protocol for CDCP1 antibody 12754-1-APDownload protocol
FC protocol for CDCP1 antibody 12754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Theranostics

Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.

Authors - Wanzun Lin
humanWB

Aging (Albany NY)

The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.

Authors - Fei Geng