验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, LNCaP cells, PC-3 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12754-1-AP targets CDCP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDCP1 fusion protein Ag3439 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CUB domain containing protein 1 |
| 别名 | Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1 |
| 计算分子量 | 836 aa, 93 kDa |
| 观测分子量 | 72 kDa, 130 kDa |
| GenBank蛋白编号 | BC021099 |
| 基因名称 | CDCP1 |
| Gene ID (NCBI) | 64866 |
| RRID | AB_2078799 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H5V8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDCP1, also called CD318, TRASK, and SIMA 135, is a 135-140 kDa transmembrane protein containing three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCP1 antibody 12754-1-AP | Download protocol |
| IHC protocol for CDCP1 antibody 12754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity. | ||
Aging (Albany NY) The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma. |
