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验证数据展示

  • IF Staining of HeLa using 67418-1-Ig (same clone as 67418-1-PBS)

    IF Staining of HeLa using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (67418-1-Ig, Clone: 2G1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • WB analysis using 67418-1-Ig (same clone as 67418-1-PBS)

    WB analysis using 67418-1-Ig (same clone as 67418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67418-1-Ig (CDCA5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDCA5 antibody included
  • IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    IHC staining of human lung cancer using 67418-1-Ig (same clone as 67418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDCA5 antibody included

CDCA5 Monoclonal antibody, PBS Only

CDCA5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67418-1-PBS

产品说明书

CloneNo. 2G1D2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CDCA5, p35, SORORIN

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDCA5 Ready-To-Use IHC Kit. CDCA5 primary antibody included.

产品信息

67418-1-PBS targets CDCA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag28958

Product name: Recombinant human CDCA5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-252 aa of BC011000

Sequence: MSGRRTRSGGAAQRSGPRAPSPTKPLRRSQRKSGSELPSILPEIWPKTPSAAAVRKPIVLKRIVAHAVEVPAVQSPRRSPRISFFLEKENEPPGRELTKEDLFKTHSVPATPTSTPVPNPEAESSSKEGELDARDLEMSKKVRRSYSRLETLGSASTSTPGRRSCFGFEGLLGAEDLSGVSPVVCSKLTEVPRVCAKPWAPDMTLPGISPPPEKQKRKKKKMPEILKTELDEWAAAMNAEFEAAEQFDLLVE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle associated 5
别名 CDCA5, p35, SORORIN
计算分子量 27 kDa
观测分子量 35 kDa
GenBank蛋白编号BC011000
基因名称 CDCA5
Gene ID (NCBI) 113130
RRIDAB_2882658
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96FF9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).

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