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Cdc7 Kinase Polyclonal antibody

Cdc7 Kinase Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17980-1-AP

Print datasheet

Synonyms

CDC7, CDC7 related kinase, CDC7L1, HsCDC7, Hsk1, huCDC7

验证数据展示

  • WB analysis of SH-SY5Y using 17980-1-AP

    WB analysis of SH-SY5Y using 17980-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17980-1-AP

    IF Staining of HepG2 using 17980-1-AP

    Immunofluorescent analysis of HepG2 cells, using CDC7 antibody 17980-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17980-1-AP targets Cdc7 Kinase in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Cdc7 Kinase fusion protein Ag12384 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 7 homolog (S. cerevisiae)
Synonyms CDC7, CDC7 related kinase, CDC7L1, HsCDC7, Hsk1, huCDC7
Calculated Molecular Weight 574 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC111044
Gene Symbol CDC7
Gene ID (NCBI) 8317
RRIDAB_2291604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
IF protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

Authors - Yiming He
View Article
humanWB

Cell Death Discov

Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening

Authors - Ling Deng
  • KD Validated
View Article