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验证数据展示

  • WB analysis using 85788-5-RR

    WB analysis using 85788-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85788-5-RR

    IF Staining of HepG2 using 85788-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85788-5-RR

    Affinity and Kinetic Characterization of 85788-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

CDC5L Recombinant antibody

CDC5L Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85788-5-RR

产品说明书

CloneNo. 250089G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U2OS cells, HCT 116 cells, NIH/3T3 cells, Neuro-2a cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85788-5-RR targets CDC5L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CDC5L fusion protein Ag3578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CDC5 cell division cycle 5-like (S. pombe)
别名 CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432
计算分子量 802 aa, 92 kDa
观测分子量100 kDa
GenBank蛋白编号BC001568
基因名称 CDC5L
Gene ID (NCBI) 988
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.

实验方案

Product Specific Protocols
WB protocol for CDC5L antibody 85788-5-RRDownload protocol
IF protocol for CDC5L antibody 85788-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols