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CDC5L Polyclonal antibody

CDC5L Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12974-1-AP

Print datasheet

Synonyms

Cdc5 like protein, CDC5L, CEF1, hCDC5, KIAA0432, PCDC5RP, Pombe cdc5 related protein

验证数据展示

  • IP experiment of mouse brain using 12974-1-AP

    IP experiment of mouse brain using 12974-1-AP

    IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.

  • WB analysis of human brain using 12974-1-AP

    WB analysis of human brain using 12974-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12974-1-AP

    IHC staining of mouse testis using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 12974-1-AP

    IF Staining of HepG2 using 12974-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12974-1-AP (CDC5L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12974-1-AP targets CDC5L in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CDC5L fusion protein Ag3578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDC5 cell division cycle 5-like (S. pombe)
Synonyms Cdc5 like protein, CDC5L, CEF1, hCDC5, KIAA0432, PCDC5RP, Pombe cdc5 related protein
Calculated Molecular Weight 802 aa, 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC001568
Gene Symbol CDC5L
Gene ID (NCBI) 988
RRIDAB_2078510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDC5L antibody 12974-1-APDownload protocol
IHC protocol for CDC5L antibody 12974-1-APDownload protocol
IF protocol for CDC5L antibody 12974-1-APDownload protocol
IP protocol for CDC5L antibody 12974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article
WB

J Biol Chem

O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.

Authors - Li Liu
View Article
humanWB

Cancer Med

Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.

Authors - Chunmei Zhang
View Article