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验证数据展示

  • IHC staining of human tonsillitis using 12427-1-AP

    IHC staining of human tonsillitis using 12427-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12427-1-AP (CDC42SE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 12427-1-AP

    IHC staining of human tonsillitis using 12427-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12427-1-AP (CDC42SE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CDC42SE1 Polyclonal antibody

CDC42SE1 Polyclonal Antibody for IHC, ELISA
Cat No. 12427-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

SPEC1, SCIP1, CDC42-binding protein SCIP1, CDC42 small effector protein 1, CDC42 small effector 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12427-1-AP targets CDC42SE1 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3098

Product name: Recombinant human CDC42SE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC012796

Sequence: MSEFWHKLGCCVVEKPQPKKKRRRIDRTMIGEPMNFVHLTHIGSGEMGAGDGLAMTGAVQEQMRSKGNRDRPWSNSRGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDC42 small effector 1
别名 SPEC1, SCIP1, CDC42-binding protein SCIP1, CDC42 small effector protein 1, CDC42 small effector 1
计算分子量 79 aa, 9 kDa
GenBank蛋白编号BC012796
基因名称 CDC42SE1
Gene ID (NCBI) 56882
RRIDAB_3669138
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRR8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC42SE1, also known as SPEC1, is a small effector protein of 9 kDa. CDC42SE1 consists of 79 amino acids, including a conserved CRIB domain, 2 conserved cysteines at the N-terminus, and a basic amino acid before the CRIB domain. CDC42SE1 has been shown to inhibit CDC42-induced JNK activity and cell morphological changes in COS1 cells (PMID: 23957315).

实验方案

Product Specific Protocols
IHC protocol for CDC42SE1 antibody 12427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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