验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11824-1-AP targets CDC42EP2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag2392 Product name: Recombinant human CDC42EP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC022337 Sequence: MSTKVPIYLKRGSRKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQGKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CDC42 effector protein (Rho GTPase binding) 2 |
| 别名 | BORG1, Cdc42 effector protein 2, CEP2 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC022337 |
| 基因名称 | CDC42EP2 |
| Gene ID (NCBI) | 10435 |
| RRID | AB_10793663 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14613 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC42EP2 (CDC42 Effector Protein 2) is a protein-coding gene that belongs to the Borg family of CDC42 effector proteins. The protein it encodes contains a CRIB (Cdc42/Rac interactive-binding) domain, which mediates its interaction with CDC42, a small Rho GTPase that regulates the formation of F-actin-containing structures. Through this binding, CDC42EP2 is thought to play a role in actin filament assembly and cell shape control, and it appears to negatively regulate CDC42 function. The gene is also known by the aliases CEP2 and BORG1, and is located on chromosome 11q12-q13. Its expression is ubiquitous but variable, with notably high levels in the heart and lower levels in the pancreas and liver.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC42EP2 antibody 11824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
