验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, K-562 cells, RT-4 cells, SiHa cells |
Planning an IHC experiment? We recommend our IHCeasy CDC42BPA Ready-To-Use IHC Kit. CDC42BPA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31297-1-AP targets CDC42BPA in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CDC42BPA fusion protein Ag34955 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CDC42 binding protein kinase alpha (DMPK-like) |
| 别名 | |
| 计算分子量 | 197 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | NM_001366019 |
| 基因名称 | CDC42BPA |
| Gene ID (NCBI) | 8476 |
| RRID | AB_3669934 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5VT25 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cdc42 binding protein kinase alpha (Cdc42BPA/MRCKα), is a member of the serine/threonine protein kinase family. Previous studies suggested that Cdc42BPA may mediates the Cdc42induced peripheral actin formation to promote cytoskeletal reorganization 5. However, the biological function and clinical significance of Cdc42BPA in colon cancer are unclear. (PMID: 29072916)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC42BPA antibody 31297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |