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验证数据展示

  • WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

  • WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse cerebellum using 67212-1-Ig (same clone as 67212-1-PBS)

    WB analysis of mouse cerebellum using 67212-1-Ig (same clone as 67212-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

CDC42 Monoclonal antibody, PBS Only

CDC42 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67212-1-PBS

产品说明书

CloneNo. 2G5E8

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

2G5E8, EC:3.6.5.2, G25K, G25K GTP binding protein, G25K GTP-binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy CDC42 Ready-To-Use IHC Kit. CDC42 primary antibody included.

产品信息

67212-1-PBS targets CDC42 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28550

Product name: Recombinant human CDC42 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-186 aa of BC002711

Sequence: MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSR

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 42 (GTP binding protein, 25kDa)
别名 2G5E8, EC:3.6.5.2, G25K, G25K GTP binding protein, G25K GTP-binding protein
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC002711
基因名称 CDC42
Gene ID (NCBI) 998
RRIDAB_2918484
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP60953
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking

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