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验证数据展示

  • WB analysis using 16293-1-AP

    WB analysis using 16293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16293-1-AP

    IHC staining of human kidney using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC37L1 antibody included
  • IHC staining of human kidney using 16293-1-AP

    IHC staining of human kidney using 16293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC37L1 antibody included
  • Featured Product
  • KD/KO验证

CDC37L1 Polyclonal antibody

CDC37L1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16293-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CDC37B, CDC37L1, HARC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human kidney tissue, Jurkat cells, LNCaP cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDC37L1 Ready-To-Use IHC Kit. CDC37L1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16293-1-AP targets CDC37L1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 CDC37L1 fusion protein Ag9459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 37 homolog (S. cerevisiae)-like 1
别名 CDC37B, CDC37L1, HARC
计算分子量 337 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC014133
基因名称 CDC37L1
Gene ID (NCBI) 55664
RRIDAB_2244711
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L3B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDC37L1 antibody 16293-1-APDownload protocol
IHC protocol for CDC37L1 antibody 16293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

J Cancer

CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.

Authors - Li Li
  • KD Validated
View Article
humanWB,IHC

Cell Death Discov

miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation.

Authors - Li Li
View Article