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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

CDC25C Monoclonal antibody, PBS Only

CDC25C Monoclonal Antibody for Indirect ELISA, WB
Cat No. 66912-1-PBS

产品说明书

CloneNo. 2B6B12

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

Indirect ELISA, WB

别名

M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66912-1-PBS targets CDC25C in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 rat, mouse, human
免疫原 CDC25C fusion protein Ag10240 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 25 homolog C (S. pombe)
别名 M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12
计算分子量 473 aa, 53 kDa
观测分子量 46-53 kDa
GenBank蛋白编号BC019089
基因名称 CDC25C
Gene ID (NCBI) 995
RRIDAB_2882239
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30307
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.