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验证数据展示

  • WB analysis of HepG2 using 55031-1-AP (same clone as 55031-1-PBS)

    WB analysis of HepG2 using 55031-1-AP (same clone as 55031-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25A antibody included
  • IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25A antibody included
  • IF Staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    IF Staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 55031-1-AP (same clone as 55031-1-PBS)

    IP experiment of HeLa using 55031-1-AP (same clone as 55031-1-PBS)

    IP result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CDC25A Polyclonal antibody, PBS Only

CDC25A Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 55031-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDC25A Ready-To-Use IHC Kit. CDC25A primary antibody included.

产品信息

55031-1-PBS targets CDC25A in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 25 homolog A (S. pombe)
别名
计算分子量 59 kDa
观测分子量 60 kDa
GenBank蛋白编号NM_001789
基因名称 CDC25A
Gene ID (NCBI) 993
RRIDAB_2881258
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30304
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

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