验证数据展示
经过测试的应用
| Positive WB detected in | MOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells |
| Positive IHC detected in | mouse colon tissue, human placenta tissue, mouse ovary tissue, rat colon tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
10683-1-AP targets CDC23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1068 Product name: Recombinant human CDC23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC005258 Sequence: MVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLVRAILKCH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 23 homolog (S. cerevisiae) |
| 别名 | ANAPC8, Anaphase-promoting complex subunit 8, APC8, Cyclosome subunit 8 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC005258 |
| 基因名称 | CDC23 |
| Gene ID (NCBI) | 8697 |
| RRID | AB_2075008 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJX2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDC23 antibody 10683-1-AP | Download protocol |
| WB protocol for CDC23 antibody 10683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC | ||
PLoS Pathog A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene. |
