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CDC20B Polyclonal antibody

CDC20B Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 13376-1-AP

Print datasheet

Synonyms

CDC20B, G6VTS76519

验证数据展示

  • IHC staining of human kidney using 13376-1-AP

    IHC staining of human kidney using 13376-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13376-1-AP (CDC20B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 13376-1-AP

    IHC staining of human kidney using 13376-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13376-1-AP (CDC20B antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 13376-1-AP

    IF Staining of HeLa using 13376-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13376-1-AP (CDC20B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13376-1-AP targets CDC20B in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC,ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CDC20B fusion protein Ag4198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 20 homolog B (S. cerevisiae)
Synonyms CDC20B, G6VTS76519
Calculated Molecular Weight 192 aa, 22 kDa
GenBank Accession NumberBC037547
Gene Symbol CDC20B
Gene ID (NCBI) 166979
RRIDAB_2877945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y33
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CDC20B antibody 13376-1-APDownload protocol
IF protocol for CDC20B antibody 13376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF,CoIP

Nat Commun

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Authors - Diego R Revinski
View Article
humanIHC

Front Oncol

Identification of Hub Genes Associated With Melanoma Development by Comprehensive Bioinformatics Analysis.

Authors - Jie Jiang
View Article
humanWB

Bioengineered

Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis.

Authors - Xumin Hou
View Article