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验证数据展示

  • WB analysis using 84531-5-RR (same clone as 84531-5-PBS)

    WB analysis using 84531-5-RR (same clone as 84531-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84531-5-RR (CDC20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

    IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC20 antibody included
  • IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

    IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC20 antibody included
  • Affinity and Kinetic Characterization of 84531-5-RR

    Affinity and Kinetic Characterization of 84531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84531-5-RR against Human CDC20 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

CDC20 Recombinant monoclonal antibody, PBS Only

CDC20 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84531-5-PBS

产品说明书

CloneNo. 241925E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

241925E12, CDC20A, p55CDC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.

产品信息

84531-5-PBS targets CDC20 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0295

Product name: Recombinant human CDC20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-211 aa of BC001088

Sequence: MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle 20 homolog (S. cerevisiae)
别名 241925E12, CDC20A, p55CDC
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC001088
基因名称 CDC20
Gene ID (NCBI) 991
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ12834
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

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