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CDAN1 Polyclonal antibody

CDAN1 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17597-1-AP

Print datasheet

Synonyms

CDA I, CDA1, CDAI, CDAN1, codanin, Codanin 1, DLT, PRO1295

验证数据展示

  • WB analysis of HeLa using 17597-1-AP

    WB analysis of HeLa using 17597-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17597-1-AP

    IP experiment of K-562 using 17597-1-AP

    IP result of anti-CDAN1 (IP:17597-1-AP, 4ug; Detection:17597-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IF Staining of HeLa using 17597-1-AP

    IF Staining of HeLa using 17597-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17597-1-AP (CDAN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 17597-1-AP is CDAN1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CDAN1 fusion protein Ag11589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name congenital dyserythropoietic anemia, type I
Synonyms CDA I, CDA1, CDAI, CDAN1, codanin, Codanin 1, DLT, PRO1295
Calculated Molecular Weight 1227 aa, 134 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC066640
Gene Symbol CDAN1
Gene ID (NCBI) 146059
RRIDAB_10694142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDAN1 acts as a negative regulator of ASF1 in chromatin assembly. It appears to play a role in nuclear envelope integrity, possibly related to microtubule attachments. Mutations of CDAN1 cause congenital dyserythropoietic anemia type I, a disease resulting in morphological and functional abnormalities of erythropoiesis. There're some isoforms this gene.

实验方案

Product Specific Protocols
WB protocol for CDAN1 antibody 17597-1-APDownload protocol
IF protocol for CDAN1 antibody 17597-1-APDownload protocol
IP protocol for CDAN1 antibody 17597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem J

A complex comprising C15ORF41 and Codanin-1: the products of two genes mutated in congenital dyserythropoietic anaemia type I (CDA-I).

Authors - Maithili Shroff
View Article