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验证数据展示

  • WB analysis of HeLa using 17597-1-AP

    WB analysis of HeLa using 17597-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17597-1-AP

    IP experiment of K-562 using 17597-1-AP

    IP result of anti-CDAN1 (IP:17597-1-AP, 4ug; Detection:17597-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IF Staining of HeLa using 17597-1-AP

    IF Staining of HeLa using 17597-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17597-1-AP (CDAN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CDAN1 Polyclonal antibody

CDAN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17597-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

Codanin 1, codanin, CDAN 1, CDAI, CDA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17597-1-AP targets CDAN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 CDAN1 fusion protein Ag11589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 congenital dyserythropoietic anemia, type I
别名 Codanin 1, codanin, CDAN 1, CDAI, CDA1
计算分子量 1227 aa, 134 kDa
观测分子量 130 kDa
GenBank蛋白编号BC066640
基因名称 CDAN1
Gene ID (NCBI) 146059
RRIDAB_10694142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDAN1 acts as a negative regulator of ASF1 in chromatin assembly. It appears to play a role in nuclear envelope integrity, possibly related to microtubule attachments. Mutations of CDAN1 cause congenital dyserythropoietic anemia type I, a disease resulting in morphological and functional abnormalities of erythropoiesis. There're some isoforms this gene.

实验方案

Product Specific Protocols
WB protocol for CDAN1 antibody 17597-1-APDownload protocol
IF protocol for CDAN1 antibody 17597-1-APDownload protocol
IP protocol for CDAN1 antibody 17597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem J

A complex comprising C15ORF41 and Codanin-1: the products of two genes mutated in congenital dyserythropoietic anaemia type I (CDA-I).

Authors - Maithili Shroff
View Article