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验证数据展示

  • IHC staining of human testis using 28169-1-AP

    IHC staining of human testis using 28169-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 28169-1-AP (CDADC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Saos-2 using 28169-1-AP

    IF Staining of Saos-2 using 28169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CDADC1 Polyclonal antibody

CDADC1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 28169-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, IF/ICC, ELISA

别名

bA103J18.1, CDADC1, NYD SP15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28169-1-AP targets CDADC1 in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag27374

Product name: Recombinant human CDADC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-188 aa of BC125202

Sequence: PAEAQRQKSQKNEEGKHGPLGDNEERTRVSTDKRQVKRTGLVVVKNMKIVGLHCSSEDLHAGQIALIKHGSRLKNCDLYFSRKPCSACLKMIVNAGVNRISYWPADPEISLLTEASSSEDAKLDAKAVERLKSNSRAHV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytidine and dCMP deaminase domain containing 1
别名 bA103J18.1, CDADC1, NYD SP15
计算分子量 514 aa, 58 kDa
GenBank蛋白编号BC125202
基因名称 CDADC1
Gene ID (NCBI) 81602
RRIDAB_3086034
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWV3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDADC1 (cytidine and dCMP deaminase domain containing 1) catalyzes the deamination of cytidine and deoxycytidine into uridine and deoxyuridine, respectively, and may play an important role in testicular development and spermatogenesis (PMID: 26945630, 16955368).

实验方案

Product Specific Protocols
IF protocol for CDADC1 antibody 28169-1-APDownload protocol
IHC protocol for CDADC1 antibody 28169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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