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验证数据展示

  • WB analysis using 28579-1-AP

    WB analysis using 28579-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28579-1-AP (CDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 28579-1-AP

    FC experiment of HeLa using 28579-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDA (28579-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CDA Polyclonal antibody

CDA Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 28579-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

cytidine deaminase, Cytidine aminohydrolase, CDD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28579-1-AP targets CDA in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CDA fusion protein Ag29507 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytidine deaminase
别名 cytidine deaminase, Cytidine aminohydrolase, CDD
计算分子量 16 kDa
观测分子量16 kDa
GenBank蛋白编号NM_001785
基因名称 CDA
Gene ID (NCBI) 978
RRIDAB_2881174
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDA antibody 28579-1-APDownload protocol
FC protocol for CDA antibody 28579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.

Authors - Congjun Zhang
View Article