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CDA Polyclonal antibody

CDA Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28579-1-AP

Print datasheet

Synonyms

CDA, CDD, Cytidine aminohydrolase, cytidine deaminase

验证数据展示

  • WB analysis using 28579-1-AP

    WB analysis using 28579-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28579-1-AP (CDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 28579-1-AP

    FC experiment of HeLa using 28579-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDA (28579-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28579-1-AP targets CDA in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CDA fusion protein Ag29507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytidine deaminase
Synonyms CDA, CDD, Cytidine aminohydrolase, cytidine deaminase
Calculated Molecular Weight 16 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberNM_001785
Gene Symbol CDA
Gene ID (NCBI) 978
RRIDAB_2881174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32320
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDA antibody 28579-1-APDownload protocol
FC protocol for CDA antibody 28579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.

Authors - Congjun Zhang
View Article