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验证数据展示

  • WB analysis using 28579-1-AP

    WB analysis using 28579-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28579-1-AP (CDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 28579-1-AP

    FC experiment of HeLa using 28579-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDA (28579-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CDA Polyclonal antibody

CDA Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 28579-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA and More (1)

别名

cytidine deaminase, Cytidine aminohydrolase, CDD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28579-1-AP targets CDA in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag29507

Product name: Recombinant human CDA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-146 aa of NM_001785

Sequence: LLTQEGRIFKGCNIENACYPLGICAERTAIQKAVSEGYKDFRAIAIASDMQDDFISPCGACRQVMREFGTNWPVYMTKPDGTYIVMTVQELLPSSFGPEDLQKTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytidine deaminase
别名 cytidine deaminase, Cytidine aminohydrolase, CDD
计算分子量 16 kDa
观测分子量16 kDa
GenBank蛋白编号NM_001785
基因名称 CDA
Gene ID (NCBI) 978
RRIDAB_2881174
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDA antibody 28579-1-APDownload protocol
FC protocol for CDA antibody 28579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.

Authors - Congjun Zhang
View Article
humanWB,IHC

Cancer Lett

Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer

Authors - Minghua Bai
View Article