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验证数据展示

  • WB analysis of mouse kidney using 84040-7-RR

    WB analysis of mouse kidney using 84040-7-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 84040-7-RR

    WB analysis of mouse testis using 84040-7-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84040-7-RR

    IHC staining of mouse brain using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84040-7-RR

    IHC staining of mouse brain using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84040-7-RR

    Affinity and Kinetic Characterization of 84040-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84040-7-RR against Mouse CD98/SLC3A2 were performed. The affinity constant is 1.36 nM.

CD98/SLC3A2 Recombinant monoclonal antibody

CD98/SLC3A2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84040-7-RR

产品说明书

CloneNo. 241247F12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse testis tissue
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84040-7-RR targets CD98/SLC3A2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1374

Product name: Recombinant Mouse CD98 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 100-526 aa of NM_008577.4

Sequence: APRCRELPVQRWWHKGALYRIGDLQAFVGRDAGGIAGLKSHLEYLSTLKVKGLVLGPIHKNQKDEINETDLKQINPTLGSQEDFKDLLQSAKKKSIHIILDLTPNYQGQNAWFLPAQADIVATKMKEALSSWLQDGVDGFQFRDVGKLMNAPLYLAEWQNITKNLSEDRLLIAGTESSDLQQIVNILESTSDLLLTSSYLSNSTFTGERTESLVTRFLNATGSQWCSWSVSQAGLLADFIPDHLLRLYQLLLFTLPGTPVFSYGDELGLQGALPGQPAKAPLMPWNESSIFHIPRPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFHALSSSPDLFSYIRHWDQNERYLVVLNFRDSGRSARLGASNLPAGISLPASAKLLLSTDSARQSREEDTSLKLENLSLNPYEGLLLQFPFVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
别名 4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1
计算分子量58 kDa
GenBank蛋白编号NM_008577.4
基因名称 CD98/SLC3A2
Gene ID (NCBI) 17254
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10852-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

实验方案

Product Specific Protocols
IHC protocol for CD98/SLC3A2 antibody 84040-7-RRDownload protocol
WB protocol for CD98/SLC3A2 antibody 84040-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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